For over 20 years Pure Protein has been a collaboration focused company working with leading researchers, companies, and institutions around the world. Our most recent work has focused on the role of HLA in antibody mediated rejection in transplantation as well as target discovery in oncology, autoimmune, and infectious diseases.
Meet Our Collaborators
“My team and I learned about Pure Protein from the positive recommendations of other researchers in the field who were using Pure Protein’s HLA products. We have been extremely satisfied with the optimal quality of these HLA molecules as well as the high professional skills of the leadership and support team. The high purity of the soluble HLA monomers together with the broad HLA repertoire have been the most valuable aspects we appreciate. It has had, and is currently having, a high impact on our project since pure sHLA is the fundamental piece of the assay we have developed to monitor circulating alloreactive (anti-HLA) memory B cells. Most importantly, we are able to use a broad HLA allele repertoire, covering both Class I and II HLA molecules, which is key for the development of our project.”
Oriol Bestard, MD, PhD
Head of the Kidney Transplant Program Bellvitge University Hospital, Barcelona
Laboratory Leader Bellvitge Research Institute (IDIBELL), Barcelona
Associate Professor, Barcelona University
“I’m a research professor and academic transplant physician at Guy’s Hospital, working to understand the role that cell-mediated immune responses play in chronic allograft dysfunction. I realized that PURE proteins could be used as a great source of antigen in ELISPOTS to measure CD4+ T cell cytokine production in response to HLA presented via the indirect pathway of allorecognition. I have also been using them to identify and characterize antigen-specific B cells. Our work has led to the development of a large program to try and suppress memory responses to HLA, which is expected to improve outcomes in sensitized individuals, and which was possible only because we had access to these highly valued reagents.”
Anthony Dorling, PhD, FRCP
Professor of Transplant Inflammation and Repair & Honorary Consultant Nephrologist Centre for Nephrology, Urology and Transplantation
“Our laboratory has had a long-term interaction with Pure Protein by using their HLA monomers for various projects. Firstly, we have used their biotinylated HLA class II monomers for the development of the first-ever HLA class II-specific memory B cell ELISPOT assay. Secondly, we have successfully used their biotinylated monomers for the fluorescence activated cell sorting of HLA-specific memory B cells from PBMC of sensitized individuals in order to make recombinant human HLA-specific monoclonal antibodies. The huge array of HLA molecules, both class I and II, that is available is essential to our projects, since we are interested in mapping the relative immunogenicity of HLA by defining the interaction between monoclonal HLA antibodies with their respective epitope.”
Sebastiaan Heidt, PhD
Accurate kinetic determinations of antigen-antibody interactions require a high degree of quality for both binding partners. Searching for functionally intact soluble HLA in order to develop and perform HLA-antibody affinity/avidity assays, I found sHLA from PureProteins to be ideally suited. Their purity, their high degree of functionality, their natural folding and their low lot to lot variability are assuring highly reliable results.
Gideon Hoenger, BSc
Senior Research Associate, Transplantation Immunology Research Laboratory, Department of Biomedicine, University Basel
and HLA Diagnostics and Immunogenetics, Laboratory Medicine, University Hospital Basel
Our research team within the Duke University Transplant Center is investigating desensitization strategies using co-stimulatory blockade to prevent the activation and differentiation of MHC-specific memory B cells into antibody secreting plasma cells. I knew the innovative scientists at Pure Protein had established high quality HLA monomers and we have recently used these monomers to interrogate HLA-specific B cell populations to specifically examine the impact of therapeutic agents on the B memory compartment.
Annette Jackson, PhD, D(ABHI)
Associate Professor in Surgery and Immunology, Duke University
Chief of Clinical Transplantation Immunology Research, Duke University
Director of the Clinical Transplantation Immunology Laboratory, Duke University
Some plasma cells may be short- or long-lived and some may be protective while others are pathogenic. As a plasma cell biologist, we identified a long-lived plasma cell compartment in the human bone marrow. In collaboration with Duke, we at Emory are interested in understanding the localization of HLA-specific plasma cells in the different compartments as well as in conventional and atypical memory B cell subsets. We are excited to use the HLA proteins from Pure Protein.
Frances Eun-Hyung Lee, MD
Associate Professor Department of Medicine, Emory University
Director of the Asthma, Allergy, and Immunology Program at The Emory Clinic Emory University
This source for HLA antigens has already been recommended to me several times by experts.Characterization of individual pre-existing or de novo donor specific HLA antibodies is becoming more and more important in this time of personalized medicine. A wide array of high quality (i.e. not denatured) HLA-monomers is therefore very useful not only for pre-transplant risk assessment but also for post-transplant monitoring.