Accelerate your research with superior assay technologies and overcome the challenge of selecting the "right" target antigen!
T Cell Epitope Mapping
Significantly improve your peptide epitope discovery process, slash development costs and strengthen your intellectual property portfolio by accessing our most advanced combinatorial peptide library screening approaches for vaccine, therapeutic and diagnostic developments.
How it Works
Select target protein, library modality and HLA alleles
Select a specific target from cancer antigens or pathogenic sequence that provides the source for your novel epitopes and choose a library modality to be specifically synthesized and screened with the HLA allele combination of your choice to identify the most potent T cell epitope candidates for your discovery program.
Receive high-throughput screening hit report
Receive primary screening results identifying candidates with affinities ranging from high to low using our state-of-the-art peptide screening assay. Moreover, the assay eliminates all non-binding peptides which need not be investigated further. The use of our technology in this primary screening will speed up your lead discovery process and deliver higher quality hits.
Prioritize top candidates for peptide epitope validation
High affinity binding is the critical factor controlling immunogenicity of peptides. Within this refinement process, prioritize the highest ranked screening hits and we validate their accurate potential by generating dose–response curves determining their inhibitory concentration (IC50) as measure of their effectiveness.
Receive a mapping report
After completion of the screens, the first epitope map for a cancer or virus-related protein can be created, showing detailed information on position of each epitope on the sequence string, their HLA-type and screening score. In addition, the map uncovers the location of immunological hotspots and cross-reactivity patterns among all HLA-molecules investigated.
Knowledge of proteins that are being targeted by the immune system is very valuable information for the development of vaccines, therapeutic interventions and diagnostics.
CANCER TARGET SELECTION
Select from the wealth of newly available genomic sequence information that provides a superb source for the identification of novel peptide epitope targets deriving from cancer antigens or other aberrant gene expressions, or from host proteins that are uniquely expressed, processed, modified during cancerous states.
INFECTIOUS DISEASE TARGET SELECTION
Select from the wealth of pathogenic sequences that provides the source for the identification of novel peptide epitope targets deriving from viral, bacterial or other pathogenic origins, or from host proteins that are uniquely expressed, processed, modified or degraded during infections.
In the discovery of new T cell epitopes, libraries of overlapping peptides can be used to identify the binding capability to a broad range of HLA alleles to select the most potent T cell epitope candidates.
TRUNCATED LIBRARY SELECTION
“Never miss an epitope!” Select a truncation library of overlapping peptides with systematic truncation of the flanking residues allowing the screening of any possible combination of 8, 9, 10, and 11-mer of a chosen target protein for a seamless HLA Class I epitope discovery assuring that no epitope is left behind to produce effective immune responses.
OVERLAP LIBRARY SELECTION
Prioritize your discovery! Our overlapping peptide library approach is used for linear, continuous epitope mapping, with the aim to generate an overlapping peptide sequence of specific length and single offset to cover the entire native protein sequence. Select the most popular libraries using 9mers or any other length of choice for your HLA Class I discovery.
Talk to an expert today
Want to know more about our Mapping Systems?
If you’re still wondering if our services are the right technology to take you to the next level and are looking for some help in defining your project needs and customize to the most relevant assay solution, reach out to our experts by visiting the Contact Us page. Someone from our team will get back to you in no time.
Expert advice, whenever you need it!
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Validate individual peptide epitope candidates by determining their physical binding characteristics and directly measure accurate IC50 values in order to judge and properly prioritize their immunogenic potential.