Enhance the value of your target protein and increase efficiency and productivity of your vaccine, therapeutic, or diagnostic developments.
Peptide Epitope Screening
Expedite your discovery process, boost the value of your sequencing data, and significantly reduce the risks of your vaccine program by prioritizing epitopes with the greatest potential and highest population coverage producing effective immune responses.
How it Works
Select a peptide and HLA allele(s)
Select from a broad panel of diverse libraries that fit your target(s) screening strategy with the intention of finding high-scoring peptide epitopes, successfully eliminating negative results, or optimizing your candidates among multiple alleles.
Select high-throughput screening mode
Option 1: 3 point screen
A 3 point screen is most often used by customers to scan large numbers of synthetic peptides with the end goal to obtain a competition score allowing a simple ranking of all candidates. The 3pt screen is primarily focused on the elimination of non-binding peptides. Peptides that exhibit binding above a predetermined threshold score are selected.
Option 2: 4 point screen
A 4 point screen generates a reduced dose-response curve that allows the approximation of logIC50 values based on a limited set of four experimental data points. It is often used by customers as a first pass evaluation to obtain more detailed data sets needed to better evaluate peptide effectiveness. Affinity categories will prioritize your logIC50 values into high, medium, or low affinity binders.
Receive data report
High affinity binding is the critical factor controlling immunogenicity of peptides. Results will be prioritized based on the highest ranked screening hits showing their HLA-type, screening score or approximate logIC50 value. Peptides with high vs low scores (logIC50) are easily identified and ranked in descending order.
Select a panel of computer predicted and pre-scored sequences and successfully eliminate false positive and negative results generated by these algorithms and produce epitope binding values and rankings that are supported by practical experience.
Select a focused or knowledge-based library of overlapping peptides by investigating smaller protein subsets that are likely to have activity at the target protein which provides a more cost effective avenue in finding a high-scoring peptide epitope candidates among multiple classes of alleles.
Select any starting set of peptide sequences whether from overlapping or separate peptide sequences, as long as the length matches the restriction of the alleles to be investigated in order to identify screening hits that are properly prioritized.
Select a restriction library to identify the restriction element of an antigen that gave rise to a vigorous T-cell response by creating an overlapping peptide set covering your target domain. Such information will be particularly useful in generating effective epitope vaccines.
Select a mutational library covering viral escape mutations in order to study the immunogenicity of different clades of viruses. This is achieved by measuring the loss of reactivity against wild-type epitopes to determine a potential sequential accumulation of CTL escape in patients during disease progression.
Select a positional or alanine scanning library for peptide optimization to refine positive screening hit series by substitution of essential amino acids in order to try to produce more potent and selective peptides which possess properties more adequate in their efficacy.
Talk to an expert today
Want to know more about our Screening Systems?
If you’re still wondering if our services are the right technology to advance your scientific discoveries and research, or are looking for some help in defining your project needs, reach out to our experts by visiting the Contact Us page. Someone from our team will get back to you in no time.
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