HLA Class II Proteins

Resources for your Immunological Research, from detecting HLA-antibody interactions to analysis of immune cells

Pure Protein’s technologically modified HLA-Class II proteins are available in soluble form at high purity. Select from a wide range of functionally intact and zipper stabilized alleles to identify, profile, or monitor antigen-specific immune responses from antibodies to immune cells.

HLA Class II molecule

HLA Class II Proteins

Resources for your Immunological Research, from detecting HLA-antibody interactions to analysis of immune cells

Pure Protein’s technologically modified HLA-Class II proteins are available in soluble form at high purity. Select from a wide range of functionally intact and zipper stabilized alleles to identify, profile, or monitor antigen-specific immune responses from antibodies to immune cells.

HLA Class II molecule

Choose from a range of HLA alleles

HLA-DQ

Select from more than 60 HLA-DQ alleles to detect and monitor humoral or cellular antigen-specific immune responses

HLA-DP

Choose from a pool of over 50 HLA-DP alleles to research the variety of heterodimeric isoforms

HLA-DR

Discover a selection of over 40 HLA-DR alleles, the emerging marker for histocompatibility screening

HLA Class II Basics

Soluble HLA (sHLA) molecules are recombinant and naturally folded glycoproteins. Using proprietary technology, Pure Protein truncates the two protein subunits just before the trans-membrane and cytoplasmic domain and stabilizes them by inserting a leucine zipper (ZP) that allows easy secretion and purification without associated cell membrane portions.

Structurally, soluble HLA class II proteins are heterodimeric glycoproteins consisting of an alpha chain (comprised of α1, α2 domains), a beta chain (comprised of β1, β2 domains) and an endogenously loaded peptide they present forming a trimeric complex.

Molecular structure of soluble HLA class II proteins
HLA Class II protein structure

Protein Characteristics

HLA Class II genes are highly polymorphic, encoding thousands of different alleles and potentially recombine to create isoform by combination of alpha and beta chains. Pure Protein offers a broad selection of single-specificity HLA proteins covering not only population frequency but also isoform availability. Like their Class I counterpart, sHLA Class II molecules also lack their transmembrane portions and are readily secreted allowing economic production and purification eliminating detergent cell lysates interferences.

Soluble Class II proteins are well characterized, proven to present native, antigenic epitopes recognized by specific antibodies, demonstrate high stability and integrity of their structures and are most compliable to most application platforms as well as highly adaptable to individual needs.

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Soluble HLA Class I Proteins

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Soluble HLA Class II Proteins

Mammalian produced, zipper stabilized, endogenously loaded, and naturally folded recombinant soluble HLA Class II proteins

Peptide Validation and Screening Services

Competition-based HLA peptide binding assays for the validation and screening of putative T cell epitopes