Engineering of HLA Peptide Complexes

HLA molecules play a critical role in many disease indications. Pure Protein produces HLA proteins in mammalian cells and deploys these HLA proteins as immunologic tools in the fields of oncology, autoimmunity, infectious disease, and transplantation. The foundation of our HLA technologies are HLA molecules that are naturally assembled and glycosylated. These natural HLA molecules are produced in large quantity for subsequent development as reagents and as diagnostics. In the field of transplantation, Pure Protein has demonstrated the serologic integrity of our HLA reagent while detecting antibodies to HLA in patient sera. Having initially developed our HLA proteins for transplant diagnostic purposes, Pure MHC next built a florescence polarization binding assay to screen large panels of candidate immune targets in the fields of infectious disease and oncology. A follow-on to this peptide binding assay was the creation of a powerful reagent whereby individual immune epitopes are loaded into any number of HLA proteins including labelled and multimerized HLA complexes. Pure Protein’s HLA-based assays and reagents demonstrate that sufficient quantities of naturally produced HLA provides our partners and collaborators with unparalleled confidence in the design and implementation of immune diagnostics and therapies.